Generuler Biotinylated DNA Ladder 生物素标记DNA分子(旧货号600-0008)
PACKAGE INSERTKPL GeneRuler™ Biotinylated DNA LadderCatalog No.Size5960-0029(600-0008)100 μLwww.seracare.comL-1003848-01June 2017508.244.6400•800.676.1881 Toll Free•508.634.3334 FaxPage 1of 1DESCRIPTIONThe KPL GeneRuler™Biotinylated DNA Ladderprovides aconvenient pre-labeled molecular weight marker ideal for useon Southern blots. The digest contains a mixture of labeled andunlabeled DNA, allowing visualization either through ethidiumbromide staining or enzymatic detection as desired. Labeledand unlabeled DNA fragments migrate uniformly.CONTENTThe ladder is supplied in 10 mM Tris-HCl/1.0 mM EDTA.Sufficient material is supplied to produce 20-50 marker lanes.STORAGE/STABILITYStore at -20°C. Stable for a minimum of one year from date ofreceipt when stored at -20°C. The ladder will remain stablethrough at least 20 freeze-thaw cycles.USEDepurination is sometimes recommended for completenicking of Genomic DNA. This will result in loss of somelabeled fragments from the ladder. Therefore, use of moreladder in procedures where depurination is required isrecommended.Gel Loading:1.Mix 2 μL (if no depurination is required) to 5 μL (ifdepurination is required) of KPL GeneRuler Biotinylated DNALadder with the appropriate volume of gel loading buffer.2.Load ladder onto an agarose gel and perform gel electrophoresisfollowing standard procedures.Following electrophoresis:1.Visualize bands by staining with ethidium bromide or SYBRgreen and view using a UV transilluminator.2.Perform transfer to nylon membrane.3.Detect using one of KPL’s Detector™ Hybridizationand Detection Kits, orby usingenzyme-labeled streptavidin andchemiluminescent orcolorimetric substrate.PRODUCT SAFETY AND HANDLINGThis product is considered non-hazardous as defined by the HazardCommunication Standard (29 CFR1910.1200). Avoid contact with skinand eyes. In case of contact or spillage, clean with copious amounts ofwater. Product may be disposed via sanitary sewer.1000080006000500040003500300025002000150012001031900800700600500400300200100The marker yields 21discrete fragments from 100 bp to 10kb. The bright bands at 3000 bp, 1031 bp and 500 bp serve as references.RELATED PRODUCTSCAT. NO.KPL Biodyne™B Nylon Membrane5960-0025(60-00-50)KPL Detector HRP Chemiluminescent Blotting Kit 5910-0027(54-30-00)KPL Detector PCR DNA Biotinylation Kit5910-0031(60-01-01)KPL Herring Sperm DNA5920-0003(60-00-14)KPL 20X SSC5150-0021(50-85-06)The product listed herein is for research use only and is not intended for usein human or clinical diagnosis. GeneRuler™is a trademark of Fermentas.